不同产地、生长年限和种质的巴戟天药材寡糖含量分析

章润菁, 李倩, 屈敏红, 高俊丽, 丁平

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (4) : 315-320.

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中国药学杂志 ›› 2016, Vol. 51 ›› Issue (4) : 315-320. DOI: 10.11669/cpj.2016.04.014
论 著

不同产地、生长年限和种质的巴戟天药材寡糖含量分析

  • 章润菁, 李倩, 屈敏红, 高俊丽, 丁平*
作者信息 +

Investigation of Morinda officinalis How Oligosaccharides from Different Habitats and Germplasm Resources with Various Ages

  • ZHANG Run-jing, LI Qian, QU Min-hong, GAO Jun-li, DING Ping*
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摘要

目的 考察37批不同产地、生长年限和种质的巴戟天药材中4种寡糖类成分的含量差异。 方法 采用HPLC-ELSD法测定上述样品中蔗糖、1-蔗果三糖、耐斯糖和1F-果呋喃耐斯糖的含量,考察不同产地、生长年限和种质对巴戟天寡糖含量的影响。结果 不同产地巴戟天药材中蔗糖、1-蔗果三糖和1F-果呋喃耐斯糖含量以广东最高,耐斯糖含量广东与福建相近,广西和海南最低;不同生长年限巴戟天药材中2.5年生巴戟天药材蔗糖和1-蔗果三糖含量最高,4年生巴戟天药材耐斯糖和1F-果呋喃耐斯糖含量最高;不同种质巴戟天药材中广东省小叶种巴戟天药材各寡糖含量远高于大叶种,同时不同种质巴戟天药材叶片形态特征也有较大差别。结论 不同产地、生长年限和种质对巴戟天药材的寡糖含量有较大影响,通过以上研究可为巴戟天药材的质量控制提供参考。

Abstract

OBJECTIVE To investigate the contents of oligosaccharides in 37 batches of Morinda officinalis How samples from different habitats and germplasm resources at various ages. METHODS HPLC-ELSD method was used to determine the contents of four oligosaccharides, i.e sucrose, 1-kestose, nystose and 1F-fructofuranosylnystose in Morinda officinalis How at different ages from different habitats and germplasm resources. The relationships among the several factors were analyzed. RESULTS The samples from Guangdong Province had larger amounts of sucrose, 1-kestose and 1F-fructofuranosylnystose than those from Fujian, Guangxi and Hainan Provinces. The content of nystose in the samples from Guangdong Province was similar with those from Fujian Province. The contents of sucrose and 1-kestose were the highest in the samples of 2.5 years old, while the contents of nystose and 1F-fructofuranosylnystose were the highest in the samples of 4 years old. The germplasm resources of small leaf had higher content of oligosaccharides than the large leaf germplasm in Guangdong Province, and different germplasm resources of Morinda officinalis How also had different morphological characteristics. CONCLUSION The contents of four Morinda officinalis How oligosaccharides vary with habitat, germplasm and age. This research may provide references for the quality control of Morinda officinalis How.

关键词

巴戟天 / 寡糖 / 产地 / 生长年限 / 种质资源

Key words

Morinda officinalis How / oligosaccharide / habitat / age / germplasm resource

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导出引用
章润菁, 李倩, 屈敏红, 高俊丽, 丁平. 不同产地、生长年限和种质的巴戟天药材寡糖含量分析[J]. 中国药学杂志, 2016, 51(4): 315-320 https://doi.org/10.11669/cpj.2016.04.014
ZHANG Run-jing, LI Qian, QU Min-hong, GAO Jun-li, DING Ping. Investigation of Morinda officinalis How Oligosaccharides from Different Habitats and Germplasm Resources with Various Ages[J]. Chinese Pharmaceutical Journal, 2016, 51(4): 315-320 https://doi.org/10.11669/cpj.2016.04.014
中图分类号: R284   

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